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Site-specific incorporation of phosphotyrosine using an expanded genetic code

Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key for understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Nat Chem Biol
Päätekijät: Hoppmann, Christian, Wong, Allison, Yang, Bing, Li, Shuwei, Hunter, Tony, Shokat, Kevan M., Wang, Lei
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2017
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5577362/
https://ncbi.nlm.nih.gov/pubmed/28604697
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2406
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