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Site-specific incorporation of phosphotyrosine using an expanded genetic code
Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key for understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog...
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| Publicado no: | Nat Chem Biol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5577362/ https://ncbi.nlm.nih.gov/pubmed/28604697 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2406 |
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