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Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy
Here we present our standard protocol for studying the binding of kinetochore proteins to microtubules as a paradigm for designing single-molecule total internal reflection fluorescence (TIRF) microscopy experiments. Several aspects of this protocol require empirical optimization, including the meth...
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| Опубликовано в: : | Cold Spring Harb Protoc |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2016
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5576995/ https://ncbi.nlm.nih.gov/pubmed/27140912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot085563 |
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