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Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy
Here we present our standard protocol for studying the binding of kinetochore proteins to microtubules as a paradigm for designing single-molecule total internal reflection fluorescence (TIRF) microscopy experiments. Several aspects of this protocol require empirical optimization, including the meth...
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| Publié dans: | Cold Spring Harb Protoc |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5576995/ https://ncbi.nlm.nih.gov/pubmed/27140912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot085563 |
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