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Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy

Here we present our standard protocol for studying the binding of kinetochore proteins to microtubules as a paradigm for designing single-molecule total internal reflection fluorescence (TIRF) microscopy experiments. Several aspects of this protocol require empirical optimization, including the meth...

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書誌詳細
出版年:Cold Spring Harb Protoc
主要な著者: Kudalkar, Emily M., Davis, Trisha N., Asbury, Charles L.
フォーマット: Artigo
言語:Inglês
出版事項: 2016
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5576995/
https://ncbi.nlm.nih.gov/pubmed/27140912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot085563
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