Llwytho...

Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy

Here we present our standard protocol for studying the binding of kinetochore proteins to microtubules as a paradigm for designing single-molecule total internal reflection fluorescence (TIRF) microscopy experiments. Several aspects of this protocol require empirical optimization, including the meth...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Cold Spring Harb Protoc
Prif Awduron: Kudalkar, Emily M., Davis, Trisha N., Asbury, Charles L.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2016
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5576995/
https://ncbi.nlm.nih.gov/pubmed/27140912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot085563
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