Llwytho...
Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy
Here we present our standard protocol for studying the binding of kinetochore proteins to microtubules as a paradigm for designing single-molecule total internal reflection fluorescence (TIRF) microscopy experiments. Several aspects of this protocol require empirical optimization, including the meth...
Wedi'i Gadw mewn:
Cyhoeddwyd yn: | Cold Spring Harb Protoc |
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Prif Awduron: | , , |
Fformat: | Artigo |
Iaith: | Inglês |
Cyhoeddwyd: |
2016
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Pynciau: | |
Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5576995/ https://ncbi.nlm.nih.gov/pubmed/27140912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot085563 |
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