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Significantly higher activity of a cytoplasmic hammerhead ribozyme than a corresponding nuclear counterpart: engineered tRNAs with an extended 3′ end can be exported efficiently and specifically to the cytoplasm in mammalian cells

Hammerhead ribozymes were expressed under the control of similar tRNA promoters, localizing transcripts either in the cytoplasm or the nucleus. The tRNA(Val)-driven ribozyme (tRNA-Rz; tRNA with extra sequences at the 3′ end) that has been used in our ribozyme studies was exported efficiently into th...

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Main Authors: Kuwabara, Tomoko, Warashina, Masaki, Koseki, Shiori, Sano, Masayuki, Ohkawa, Jun, Nakayama, Kazuhisa, Taira, Kazunari
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2001
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC55762/
https://ncbi.nlm.nih.gov/pubmed/11433023
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