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Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips

A generic hexadeoxyribonucleotide microchip has been applied to test the DNA-binding properties of HU histone-like bacterial protein, which is known to have a low sequence specificity. All 4096 hexamers flanked within 8mers by degenerate bases at both the 3′- and 5′-ends were immobilized within the...

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Autori principali: Krylov, Alexander S., Zasedateleva, Olga A., Prokopenko, Dmitry V., Rouviere-Yaniv, Josette, Mirzabekov, Andrei D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC55728/
https://ncbi.nlm.nih.gov/pubmed/11410675
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