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DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1

The fidelity of the yeast retrotransposon Ty1 reverse transcriptase (RT) was determined by an assay based on gel electrophoresis. Steady-state kinetics analyses of deoxyribonucleotide (dNTP) incorporation at a defined primer-template site indicate that Ty1 RT misincorporates dNTP at a frequency of 0...

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Pubblicato in:Nucleic Acids Res
Autori principali: Boutabout, Mansour, Wilhelm, Marcelle, Wilhelm, François-Xavier
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2001
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55714/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11376139/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.11.2217
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