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DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1

The fidelity of the yeast retrotransposon Ty1 reverse transcriptase (RT) was determined by an assay based on gel electrophoresis. Steady-state kinetics analyses of deoxyribonucleotide (dNTP) incorporation at a defined primer-template site indicate that Ty1 RT misincorporates dNTP at a frequency of 0...

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Main Authors: Boutabout, Mansour, Wilhelm, Marcelle, Wilhelm, François-Xavier
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2001
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC55714/
https://ncbi.nlm.nih.gov/pubmed/11376139
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