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A Systematic Protein Refolding Screen Method using the DGR Approach Reveals that Time and Secondary TSA are Essential Variables

Refolding of proteins derived from inclusion bodies is very promising as it can provide a reliable source of target proteins of high purity. However, inclusion body-based protein production is often limited by the lack of techniques for the detection of correctly refolded protein. Thus, the selectio...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wang, Yuanze, van Oosterwijk, Niels, Ali, Ameena M., Adawy, Alaa, Anindya, Atsarina L., Dömling, Alexander S. S., Groves, Matthew R.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5570958/
https://ncbi.nlm.nih.gov/pubmed/28839267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-09687-z
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