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Enzymatic properties of rat DNA polymerase β mutants obtained by randomized mutagenesis

We have used random sequence mutagenesis to generate mutants of DNA polymerase β in an effort to identify amino acid residues important for function, catalytic efficiency and fidelity of replication. A library containing 100 000 mutants at residues 274–278 in the N-helix of the thumb subdomain of th...

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Detalhes bibliográficos
Main Authors: Skandalis, Adonis, Loeb, Lawrence A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC55708/
https://ncbi.nlm.nih.gov/pubmed/11376161
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