A dual role for substrate S-adenosyl-l-methionine in the methylation reaction with bacteriophage T4 Dam DNA-[N6-adenine]-methyltransferase

The fluorescence of 2-aminopurine ((2)A)-substituted duplexes (contained in the GATC target site) was investigated by titration with T4 Dam DNA-(N6-adenine)-methyltransferase. With an unmethylated target ((2)A/A duplex) or its methylated derivative ((2)A/(m)A duplex), T4 Dam produced up to a 50-fold...

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Main Authors: Malygin, Ernst G., Evdokimov, Alexey A., Zinoviev, Victor V., Ovechkina, Lidiya G., Lindstrom, William M., Reich, Norbert O., Schlagman, Samuel L., Hattman, Stanley
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC55703/
https://ncbi.nlm.nih.gov/pubmed/11376154
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