Binding of the IS903 transposase to its inverted repeat in vitro.

We have purified the transposase of IS903 in three different ways. We find that transposase expressed as a fusion protein with either glutathione-S-transferase or maltose-binding protein is soluble and can be purified rapidly using affinity chromatography. The third purification requires extracting...

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Bibliographic Details
Main Authors: Derbyshire, K M, Grindley, N D
Format: Artigo
Language:Inglês
Published: 1992
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC556880/
https://ncbi.nlm.nih.gov/pubmed/1324175
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