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Bicarbonate alters cellular responses in respiration assays
Metabolic assay buffers often omit bicarbonate, which is susceptible to alkalinisation in an open environment. Here, we assessed the effect of including bicarbonate in respirometry experiments. By supplementing HEPES-buffered media with low concentrations of bicarbonate, we found increased respirati...
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| Publié dans: | Biochem Biophys Res Commun |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5568524/ https://ncbi.nlm.nih.gov/pubmed/28559140 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2017.05.151 |
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