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Bicarbonate alters cellular responses in respiration assays

Metabolic assay buffers often omit bicarbonate, which is susceptible to alkalinisation in an open environment. Here, we assessed the effect of including bicarbonate in respirometry experiments. By supplementing HEPES-buffered media with low concentrations of bicarbonate, we found increased respirati...

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書目詳細資料
發表在:Biochem Biophys Res Commun
Main Authors: Krycer, James R., Fisher-Wellman, Kelsey H., Fazakerley, Daniel J., Muoio, Deborah M., James, David E.
格式: Artigo
語言:Inglês
出版: 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5568524/
https://ncbi.nlm.nih.gov/pubmed/28559140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2017.05.151
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