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Secretory expression and purification of Bacillus licheniformis keratinase in insect cells
The keratinase (kerA) gene from Bacillus licheniformis PWD-1 was expressed and purified in insect cells. First, the sequence encoding Ker-His-Flag was designed based on the amino acid sequence of the protein and peptide and codon optimization in order to ensure the high expression in insect cells. I...
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| Vydáno v: | PLoS One |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Public Library of Science
2017
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5568435/ https://ncbi.nlm.nih.gov/pubmed/28832667 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183764 |
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