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Secretory expression and purification of Bacillus licheniformis keratinase in insect cells

The keratinase (kerA) gene from Bacillus licheniformis PWD-1 was expressed and purified in insect cells. First, the sequence encoding Ker-His-Flag was designed based on the amino acid sequence of the protein and peptide and codon optimization in order to ensure the high expression in insect cells. I...

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Bibliografski detalji
Izdano u:PLoS One
Glavni autori: Huang, Miaorong, Chen, Ruiai, Ren, Guangcai
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5568435/
https://ncbi.nlm.nih.gov/pubmed/28832667
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183764
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