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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Most proteins act in association with others; hence, it is crucial to characterize these functional units in order to fully understand biological processes. Affinity purification coupled to mass spectrometry (AP-MS) has become the method of choice for identifying protein-protein interactions. Howeve...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Pardo, Mercedes, Bode, Daniel, Yu, Lu, Choudhary, Jyoti S.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5564443/
https://ncbi.nlm.nih.gov/pubmed/28447986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55498
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