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Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Accurate detection and identification of low frequency mutations can be problematic when assessing residual disease after therapy, screening for emerging resistance mutations during therapy, or when patients have few circulating tumor cells. Wild-type blocking PCR followed by sequencing analysis off...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Albitar, Adam Z., Ma, Wanlong, Albitar, Maher
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5564440/
https://ncbi.nlm.nih.gov/pubmed/28447972
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55130
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