CrY2H-seq: a massively-multiplexed assay for deep coverage interactome mapping

Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively-multiplexed yeast two-hybrid method, CrY2H-seq, that uses a Cre recombinase interaction reporter to intracellularly fuse the c...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Wanamaker, Shelly A., Garza, Renee M., MacWilliams, Andrew, Nery, Joseph R., Bartlett, Anna, Castanon, Rosa, Goubil, Adeline, Feeney, Joseph, O’Malley, Ronan, Huang, Shao-shan Carol, Zhang, Zhuzhu Z., Galli, Mary, Ecker, Joseph R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5564216/
https://ncbi.nlm.nih.gov/pubmed/28650476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4343
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