Color-blind fluorescence detection for four-color DNA sequencing

We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing. To fully demonstrate the advantages of PME, a fluorescent dye set has been developed whose absorpt...

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Detalhes bibliográficos
Main Authors: Lewis, Ernest K., Haaland, Wade C., Nguyen, Freddy, Heller, Daniel A., Allen, Matthew J., MacGregor, Robert R., Berger, C. Scott, Willingham, Britain, Burns, Lori A., Scott, Graham B. I., Kittrell, Carter, Johnson, Bruce R., Curl, Robert F., Metzker, Michael L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2005
Assuntos:
Physical Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC556273/
https://ncbi.nlm.nih.gov/pubmed/15800037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0501606102
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