Color-blind fluorescence detection for four-color DNA sequencing

We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing. To fully demonstrate the advantages of PME, a fluorescent dye set has been developed whose absorpt...

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Détails bibliographiques
Auteurs principaux: Lewis, Ernest K., Haaland, Wade C., Nguyen, Freddy, Heller, Daniel A., Allen, Matthew J., MacGregor, Robert R., Berger, C. Scott, Willingham, Britain, Burns, Lori A., Scott, Graham B. I., Kittrell, Carter, Johnson, Bruce R., Curl, Robert F., Metzker, Michael L.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2005
Sujets:
Physical Sciences
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC556273/
https://ncbi.nlm.nih.gov/pubmed/15800037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0501606102
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