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Defining the Structural Basis for Allosteric Product Release from E. coli Dihydrofolate Reductase Using NMR Relaxation Dispersion

[Image: see text] The rate-determining step in the catalytic cycle of E. coli dihydrofolate reductase is tetrahydrofolate (THF) product release, which can occur via an allosteric or an intrinsic pathway. The allosteric pathway, which becomes accessible when the reduced cofactor NADPH is bound, invol...

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Detalhes bibliográficos
Publicado no:J Am Chem Soc
Main Authors: Oyen, David, Fenwick, R. Bryn, Aoto, Phillip C., Stanfield, Robyn L., Wilson, Ian A., Dyson, H. Jane, Wright, Peter E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2017
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5562155/
https://ncbi.nlm.nih.gov/pubmed/28737940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b05958
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