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A promiscuous split intein with expanded protein engineering applications

The protein trans-splicing (PTS) activity of naturally split inteins has found widespread use in chemical biology and biotechnology. However, currently used naturally split inteins suffer from an “extein dependence,” whereby residues surrounding the splice junction strongly affect splicing efficienc...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Stevens, Adam J., Sekar, Giridhar, Shah, Neel H., Mostafavi, Anahita Z., Cowburn, David, Muir, Tom W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5559002/
https://ncbi.nlm.nih.gov/pubmed/28739907
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1701083114
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