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Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor
We describe the development of an optimized glycolytic flux biosensor and its application in detecting altered flux in a production strain and in a mutant library. The glycolytic flux biosensor is based on the Cra-regulated ppsA promoter of E. coli controlling fluorescent protein synthesis. We valid...
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| Publicado no: | Metab Eng |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Academic Press
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5555440/ https://ncbi.nlm.nih.gov/pubmed/28709932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymben.2017.07.002 |
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