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Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems

Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different...

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Bibliografische gegevens
Gepubliceerd in:PLoS One
Hoofdauteurs: Saiz-Baggetto, Sara, Méndez, Ester, Quilis, Inma, Igual, J. Carlos, Bañó, M. Carmen
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2017
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5553802/
https://ncbi.nlm.nih.gov/pubmed/28800621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183067
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