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Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems
Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5553802/ https://ncbi.nlm.nih.gov/pubmed/28800621 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183067 |
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