Site-directed in vitro replacement of nucleosides in the anticodon loop of tRNA: application to the study of structural requirements for queuine insertase activity.

We have investigated the specificity of the enzymes Q-insertase and mannosyl-Q transferase that replace the guanosine at position 34 (wobble base) in the anticodon of several tRNAs by Q or mannosyl-Q derivatives. We have restructured in vitro the normal anticodon of yeast tRNA-Asp-GUC, yeast tRNAArg...

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Autores principales: Carbon, P, Haumont, E, Fournier, M, de Henau, S, Grosjean, H
Formato: Artigo
Lenguaje:Inglês
Publicado: 1983
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC555240/
https://ncbi.nlm.nih.gov/pubmed/6354708
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