FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry

We describe a flow-cytometry-based protocol for intracellular mRNA measurements in nonadherent mammalian cells using fluorescence in situ hybridization (FISH) probes. The method, which we call FISH-Flow, allows for high-throughput multiparametric measurements of gene expression, a task that was not...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Arrigucci, Riccardo, Bushkin, Yuri, Radford, Felix, Lakehal, Karim, Vir, Pooja, Pine, Richard, Martin, December, Sugarman, Jeffrey, Zhao, Yanlin, Yap, George S, Lardizabal, Alfred A, Tyagi, Sanjay, Gennaro, Maria Laura
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5548662/
https://ncbi.nlm.nih.gov/pubmed/28518171
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.039
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