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Adenylate cyclase from bovine brain cortex: purification and characterization of the catalytic unit.
The non-stimulated (basal) adenylate cyclase from bovine brain cortical membranes was purified 10 000-fold to apparent homogeneity by Lubrol PX extraction and two cycles of affinity chromatography on forskolin-agarose. The final product appears as one major band (mol. wt. 115 000) on SDS-polyacrylam...
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| Publicado no: | EMBO J |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554717/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4092692/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb04134.x |
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