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Properties of the separated catalytic and regulatory units of brain adenylate cyclase.

Adenylate cyclase from bovine brain cortex was solubilized with 14 mM cholate and 1 M (NH4)2SO4. Gel filtration over a column of Sepharose 6B separated the catalytic unit (CU) from a factor (G/F) that confers responsiveness to 5'-guanylyl imidophosphate (p[NH]ppG) or fluoride. The separated CU,...

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Detalhes bibliográficos
Main Authors: Strittmatter, S, Neer, E J
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC350280/
https://ncbi.nlm.nih.gov/pubmed/6935648
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