Liposome-mediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid.

An Escherichia coli plasmid, pLGV23neo, carrying a kanamycin resistance gene expressed in plant cells, was encapsulated into negatively charged liposomes prepared by the reverse phase evaporation technique. These liposomes were induced to fuse with tobacco mesophyll protoplasts by polyethyleneglycol...

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Bibliographic Details
Main Authors: Deshayes, A, Herrera-Estrella, L, Caboche, M
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC554571/
https://ncbi.nlm.nih.gov/pubmed/3905385
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