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Liposome-mediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid.
An Escherichia coli plasmid, pLGV23neo, carrying a kanamycin resistance gene expressed in plant cells, was encapsulated into negatively charged liposomes prepared by the reverse phase evaporation technique. These liposomes were induced to fuse with tobacco mesophyll protoplasts by polyethyleneglycol...
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| Publicado no: | EMBO J |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554571/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3905385/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03996.x |
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