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Liposome-mediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid.
An Escherichia coli plasmid, pLGV23neo, carrying a kanamycin resistance gene expressed in plant cells, was encapsulated into negatively charged liposomes prepared by the reverse phase evaporation technique. These liposomes were induced to fuse with tobacco mesophyll protoplasts by polyethyleneglycol...
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| Pubblicato in: | EMBO J |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group
1985
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554571/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3905385/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03996.x |
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