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Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator

The graTA operon from Pseudomonas putida encodes a toxin–antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in...

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Publicat a:Acta Crystallogr F Struct Biol Commun
Autors principals: Talavera, Ariel, Tamman, Hedvig, Ainelo, Andres, Hadži, San, Garcia-Pino, Abel, Hõrak, Rita, Konijnenberg, Albert, Loris, Remy
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5544002/
https://ncbi.nlm.nih.gov/pubmed/28777088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X17009438
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