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Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator
The graTA operon from Pseudomonas putida encodes a toxin–antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in...
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| Publicado no: | Acta Crystallogr F Struct Biol Commun |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5544002/ https://ncbi.nlm.nih.gov/pubmed/28777088 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X17009438 |
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