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Transformation of Saccharomyces cerevisiae and Schizosaccharomyces pombe with linear plasmids containing 2 micron sequences.

Linear plasmids were constructed by adding telomeres prepared from Tetrahymena pyriformis rDNA to a circular hybrid Escherichia coli-yeast vector and transforming Saccharomyces cerevisiae. The parental vector contained the entire 2 mu yeast circle and the LEU gene from S. cerevisiae. Three transform...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Guerrini, A M, Ascenzioni, F, Tribioli, C, Donini, P
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1985
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC554383/
https://ncbi.nlm.nih.gov/pubmed/3896773
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