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Deletion of plasmid sequences during Saccharomyces cerevisiae transformation.

Saccharomyces cerevisiae was transformed with DNA by the lithium acetate method. Mutation of nonselected markers on the transforming vector was observed at a frequency several orders of magnitude higher than spontaneous mutation frequencies. These mutations were shown to be deletions. Linearization...

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Detalhes bibliográficos
Main Authors: Clancy, S, Mann, C, Davis, R W, Calos, M P
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC215771/
https://ncbi.nlm.nih.gov/pubmed/6090387
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