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Deletion of plasmid sequences during Saccharomyces cerevisiae transformation.

Saccharomyces cerevisiae was transformed with DNA by the lithium acetate method. Mutation of nonselected markers on the transforming vector was observed at a frequency several orders of magnitude higher than spontaneous mutation frequencies. These mutations were shown to be deletions. Linearization...

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Vydáno v:J Bacteriol
Hlavní autoři: Clancy, S, Mann, C, Davis, R W, Calos, M P
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1984
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215771/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6090387/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.3.1065-1067.1984
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