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Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.

A phoE-lacZ hybrid gene encoding the N-terminal 300 amino acid residues of pre-PhoE protein, fused to an almost complete beta-galactosidase molecule was constructed in vitro. Cell fractionation experiments suggested that the hybrid gene product is transported to the outer membrane. However, by using...

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Main Authors: Tommassen, J, Leunissen, J, van Damme-Jongsten, M, Overduin, P
Format: Artigo
Jezik:Inglês
Izdano: 1985
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC554297/
https://ncbi.nlm.nih.gov/pubmed/2990905
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