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Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.

A phoE-lacZ hybrid gene encoding the N-terminal 300 amino acid residues of pre-PhoE protein, fused to an almost complete beta-galactosidase molecule was constructed in vitro. Cell fractionation experiments suggested that the hybrid gene product is transported to the outer membrane. However, by using...

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Detalles Bibliográficos
Publicado en:EMBO J
Principais autores: Tommassen, J, Leunissen, J, van Damme-Jongsten, M, Overduin, P
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 1985
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554297/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2990905/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03736.x
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