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A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
The construction and use of a Tn3-lac transposon, Tn3-HoHo1, is described. Tn3-HoHo1 can serve as a transposon mutagen and provides a new and useful system for the random generation of both transcriptional and translational lacZ gene fusions. In these fusions the production of beta-galactosidase, th...
Enregistré dans:
| Publié dans: | EMBO J |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
1985
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554276/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2990912/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03715.x |
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