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A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.

The construction and use of a Tn3-lac transposon, Tn3-HoHo1, is described. Tn3-HoHo1 can serve as a transposon mutagen and provides a new and useful system for the random generation of both transcriptional and translational lacZ gene fusions. In these fusions the production of beta-galactosidase, th...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Stachel, S E, An, G, Flores, C, Nester, E W
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554276/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2990912/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03715.x
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