Highly reproducible improved label-free quantitative analysis of cellular phosphoproteome by optimization of LC-MS/MS gradient and analytical column construction

Expanding the sequencing depth of the peptides with a statistically significant quantitative change derived from a biological stimulation is critical. Here we demonstrate that optimization of LC gradient and analytical column construction can reveal over 30,000 unique peptides and 23,000 phosphopept...

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Udgivet i:J Proteomics
Main Authors: Ahsan, Nagib, Belmont, Judson, Chen, Zhuo, Clifton, James G., Salomon, Arthur R.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5542054/
https://ncbi.nlm.nih.gov/pubmed/28634120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2017.06.013
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