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Effects of Column and Gradient Lengths on Peak Capacity and Peptide Identification in nanoflow LC-MS/MS of Complex Proteomic Samples

Reversed-phase liquid chromatography is the most commonly used separation method for shotgun proteomics. Nanoflow chromatography has emerged as the preferred chromatography method for its increased sensitivity and separation. Despite its common use, there are a wide range of parameters and condition...

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Detalhes bibliográficos
Main Authors: Hsieh, Edward J., Bereman, Michael S., Durand, Stanley, Valaskovic, Gary A., MacCoss, Michael J
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3554873/
https://ncbi.nlm.nih.gov/pubmed/23197307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0508-6
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