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Production of thiol-penicillin-binding protein 3 of Escherichia coli using a two primer method of site-directed mutagenesis.

The active site serine residue of penicillin-binding protein 3 of Escherichia coli that is acylated by penicillin (Ser-307) has been converted to a cysteine residue using a simple and efficient two primer method of site-directed mutagenesis. The resulting thiol-penicillin-binding protein 3 was expre...

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Библиографические подробности
Главные авторы: Broome-Smith, J K, Hedge, P J, Spratt, B G
Формат: Artigo
Язык:Inglês
Опубликовано: 1985
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC554174/
https://ncbi.nlm.nih.gov/pubmed/3894005
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