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gal4 transcription activator protein of yeast can function as a repressor in Escherichia coli.

The chromosomal lac operator of Escherichia coli was replaced by a 22 bp oligonucleotide containing the binding site of the yeast gal4 protein. Induction of gal4 protein synthesis in these bacteria repressed beta-galactosidase synthesis at least 30-fold. These results show that it is possible to det...

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Bibliografische gegevens
Gepubliceerd in:EMBO J
Hoofdauteurs: Paulmier, N, Yaniv, M, von Wilcken-Bergmann, B, Müller-Hill, B
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 1987
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553814/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3123219/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1987.tb02680.x
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