Perforated MDCK cells support intracellular transport.

We have developed a method for perforating the plasma membrane of MDCK cells while retaining cellular functions. A nitrocellulose acetate filter was applied to the apical side of cells, grown on a glass coverslip, and allowed to dry. Segments of the apical plasma membrane adhered to the filter and w...

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Bibliographic Details
Published in:EMBO J
Main Authors: Simons, K, Virta, H
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 1987
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553624/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3665874/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1987.tb02496.x
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