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Exocytic transport vesicles generated in vitro from the trans-Golgi network carry secretory and plasma membrane proteins.

We have developed a cell-free assay that reproduces vesicular budding during exit from the Golgi complex. The starting preparation for the in vitro system was a rat liver stacked Golgi fraction immobilized on a magnetic solid support by means of an antibody against the cytoplasmic domain of the poly...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Salamero, J, Sztul, E S, Howell, K E
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54819/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2217204/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.19.7717
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