Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.

We have used a novel approach to investigate the control of initiation of replication of sperm nuclei in a Xenopus cell-free extract. Nascent DNA was labelled with biotin by supplementing the extract with biotin-11-dUTP, and isolated nuclei were then probed with fluorescein-conjugated streptavidin....

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Detalhes bibliográficos

Publicado no:	EMBO J
Principais autores:	Blow, J J, Watson, J V
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Nature Publishing Group 1987
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553588/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3653079/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1987.tb02463.x
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